MALDI MS Imaging-Molecular Mapping of Biological Samples by MALDI Mass Spectrometry

Atmospheric pressure molecular imaging by infrared MALDI mass spectrometry.

An atmospheric pressure (AP) MALDI imaging interface was developed for an orthogonal acceleration time-of-flight mass spectrometer and utilized to analyze peptides, carbohydrates, and other small biomolecules using infrared laser excitation. In molecular imaging experiments, the spatial distribution of mock peptide patterns was recovered with a detection limit of approximately 1 fmol/pixel from...

MALDI mass spectrometry imaging in oncology (Review).

Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) has improved over the years and is increasingly being used for biomarker discovery directly from human tissue sections. State-of-the-art technology currently enables a resolution down to 20 µm. MSI therefore allows the correlation of spatial and temporal protein expression profiles with distinct morphological fe...

Characterization of fengycin homologues produced by Bacillus subtilis K1 by MALDI-TOF tandem mass spectrometry (MALDI TOF MS/MS)

Mass Microscope for MALDI Molecular Imaging in Biological Tissue Sections

Mass microscope combined with matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is applied for imaging of biomolecules due to the high resolution of microscopic lens present in the instrument for scanning the tissue sections at the level of micro meter (μm) to reveal the distribution of molecules in samples. Therefore, the MALD-IMS is a powerful tool in investiga...

Imaging of lipid species by MALDI mass spectrometry.

Recent developments in MALDI have enabled direct detection of lipids as intact molecular species present within cellular membranes. Abundant lipid-related ions are produced from the direct analysis of thin tissue slices when sequential spectra are acquired across a tissue surface that has been coated with a MALDI matrix. The lipid-derived ions can often be distinguished from other biomolecules ...